000			02441nam a2200193 4500
999			_c12949 _d12949
082			_aRef 373.07 G8201 2024
245			_aGreen synthesis of silver nanoparticles using alagaw (Premma odorata) leaves extract and its antibacterial activity against Escherichia coli / _cby Julianna Marie A. Basinang, Samantha Gale P. Gemarino, Dranel Genesis S. Grecia, & Gabriel Anthony A. Tinsay.
260			_a[Iloilo City] : _b[Ateneo de Iloilo], _cc2024.
300			_a59 p.
520			_aEscherichia coli poses a significant public health threat in the Philippines, with a total of 10,451 reported cases during 2022. Green synthesis of silver nanoparticles exhibits significant antimicrobial actions to bacterial infections. Alagaw (Premna odorata) has been reported to possess antibacterial properties. Thus, this study generally aims to determine the antibacterial activity of the synthesized silver nanoparticles using Alagaw leaves extract against E. coli. This study utilized 200 g of Alagaw leaves extracted using 95% ethanol and used to synthesize silver nanoparticles. The synthesized silver nanoparticles were then subjected to Ultraviolet Spectrometer for characterization and tested for antibacterial activity through zone inhibition using Disk diffusion method after 18-24 hours of incubation. The treatments were replicated thrice and are as follows: Treatment A - 5μl, B- 10μl, C - 20μl of green synthesized silver nanoparticles from Alagaw leaves extract, positive control (Ciprofloxacin) and negative control (Sterile Ultrapure Water). The mean was determined using Kruskal-Wallis test and computed at 5% level of significance. Results revealed that the positive control (45 mm), got the best result followed by C (13. 33 mm), B (10.33 mm), A (10 mm), and the negative control (o mm). Kruskal- Wallis results revealed that the p-value (0.011) is less than 5% level of significance, therefore the null hypothesis was rejected. Hence, there is a significant difference. Based on the results gathered, it is concluded that the synthesized silver nanoparticles using Alagaw leaves extract have the potential to inhibit the growth of E. coli.
526			_a300-399
650			_aAntibacterial agents--Research.
650			_aPremna odorata.
700			_aBasinang, Julianna Marie A.
700			_aGemarino, Samantha Gale P.
700			_aGrecia, Dranel Genesis S.
700			_aTinsay, Gabriel Anthony A.
942			_2ddc _cBK